Estimates of Autozygosity Through Runs of Homozygosity in Farmed Coho Salmon.

The characterization of runs of homozygosity (ROH), using high-density single nucleotide polymorphisms (SNPs) allows inferences to be made about the past demographic history of animal populations and the genomic ROH has become a common approach to characterize the inbreeding.

We aimed to analyze and characterize ROH patterns and compare different genomic and pedigree-based methods to estimate the inbreeding coefficient in two pure lines (POP A and B) and one recently admixed line (POP C) of coho salmon (Oncorhynchus kisutch) breeding nuclei, genotyped using a 200 K Affymetrix Axiom^® myDesign Custom SNP Array.

A large number and greater mean length of ROH were found for the two “pure” lines and the recently admixed line (POP C) showed the lowest number and smaller mean length of ROH. The ROH analysis for different length classes suggests that all three coho salmon lines the genome is largely composed of a high number of short segments (<4 Mb), and for POP C no segment >16 Mb was found.

TBS buffer (Tris-Buffered Saline), 10x Solution
UA00279	Bio Basic	500ml	EUR 58.48

TBS buffer (Tris-Buffered Saline), 10x Solution
A0027	Bio Basic	4L	EUR 93.93

Tris Buffered Saline (TBS) 10x pH 8.0, 1000 ml
12-9134-10	Medicago	1000 ml	EUR 459

TBS
I004	Cygnus Technologies	1000 ml	EUR 219
Description: TBS by Cygnus Technologies is available in Europe via Gentaur.

T-Pro Washing buffer in TBS and Tween-20 (10X)
JB09-I004	T-Pro Biotechnology	500ml/BT	EUR 144

T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing
JB09-I002	T-Pro Biotechnology	500ml/BT	EUR 135

Tris Buffered Saline (TBS, pH 7.4) concentrate (10X, 100 mM Tris/150mM NaCl final)
80082	Alpha Diagnostics	1 L	EUR 225

Tris Buffered Saline (TBS, pH 7.4) concentrate (10X, 50 mM Tris/150mM NaCl final)
80083	Alpha Diagnostics	1 L	EUR 225

SkipDewax, 10X
T213	Insitus Biotechnologies	50ml	EUR 297

PBS, 10X
UPD8117	Bio Basic	500ml	EUR 65.66

TBS Wash Concentrate, 20X
F004	Cygnus Technologies	50 ml	EUR 180
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur.

TBS Wash Concentrate, 20X
F004-1000	Cygnus Technologies	1000 ml	EUR 403
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur.

TBS Blocking Buffer Pack
AR0143	BosterBio	200mL/pack	EUR 65

TBS Wash Buffer Pack
AR0144	BosterBio	500mL/pack	EUR 60

EZBlock? (TBS) Blocking Buffer
2117-1000	Biovision		EUR 212

EZBlock? (TBS) Blocking Buffer
2117-200	Biovision		EUR 125

THROMBOSTEP
TBS-25	ImmunoStep	25 test	EUR 778

THROMBOSTEP
TBS-50	ImmunoStep	50 test	EUR 986

TBS blocking buffer with BSA
SD8113	Bio Basic	1pk, 1L	EUR 83.93

Tris Buffered Saline (TBS) Powder
AR0031	BosterBio	2000mL/pack	EUR 53

TBS Buffer, 20X pH 7.4
T8054-050	GenDepot	500ml	EUR 86

TBS Buffer, 20X pH 7.4
T8054-101	GenDepot	1L	EUR 99

TBS Buffer, 20X pH 7.6
T8055-050	GenDepot	500ml	EUR 86

TBS Buffer, 20X pH 7.6
T8055-101	GenDepot	1L	EUR 99

EZBlock? T20 (TBS) Blocking Buffer
2140-1000	Biovision		EUR 218

EZBlock? T20 (TBS) Blocking Buffer
2140-200	Biovision		EUR 120

TissueDigest Diluent, 10X
T102	Insitus Biotechnologies	10ml	EUR 94

450ML PBS 10X
NAT1006	National Diagnostics	450ML	EUR 80

4L PBS 10X
NAT1008	National Diagnostics	4L	EUR 199

1L TBE 10X
NAT1196	National Diagnostics	1L	EUR 82

4L TBE 10X
NAT1198	National Diagnostics	4L	EUR 121

10X Loading Buffer
L0503-005	GenDepot	5X1ml	EUR 77

10X Loading Buffer
L0504-005	GenDepot	5X1ml	EUR 77

10X MOPS Buffer
M1057-050	GenDepot	500ml	EUR 161

10X MOPS Buffer
M1057-100	GenDepot	2X500ml	EUR 219

NH4 Buffer, 10x
BIO-37025	Bioline	3 x 1.2ml	Ask for price

Trypsin, 2.5%(10X)
CA019-010	GenDepot	100ml	EUR 80

Collagenase/Hyaluronidase (10X)
CA094-002	GenDepot	20ml	EUR 340

10x Taq Buffer
BA00151	Bioatlas	1.8ml	EUR 55
Description: High purity buffer for various PCR applications.

10X TAE Buffer
T8048-101	GenDepot	1L	EUR 89

10x Proteinase K
TG4039	TopoGen	0.5ml	EUR 134

PBS (10X Concentrate)
85R-125	Fitzgerald	250 ml	EUR 235
Description: Phosphate Buffered Saline (PBS) 10X concentrate

TBS with non fat milk premix
SD8104	Bio Basic	1pk	EUR 63.49

TBST (TBS-Tween 20), 10XpH 7.6
UA0046	Bio Basic	500ml	EUR 69.93

ChonBlock Western Blot Buffer 1 (TBS)
9069T	Chondrex	10 ml	EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (TBS)

Casein-based TBS Blocking Buffer Pack
AR0183	BosterBio	500mL/Pack	EUR 70

10 x TBS (Tris-buffered saline)
GR103023	Genorise Scientific	1 L	EUR 89

Blocking Buffer, 10X, 250ML
X109-250ML	Arbor Assays	250ML	EUR 359

Blocking Buffer, 10X, 25ML
X109-25ML	Arbor Assays	25ML	EUR 122

10x Taq Buffer (MgCl2)
PCRB16	Bio Basic	4x1.5ml, 6ml	EUR 58.7

Tissue Pre-Conditioner, 10X
T201	Insitus Biotechnologies	50ml	EUR 297

SkipDewax Trial Size, 10X
T212	Insitus Biotechnologies	10ml	EUR 123

25ML 10X CE Buffer
NAT1206	National Diagnostics	25ML	EUR 115

100ML 10X CE Buffer
NAT1208	National Diagnostics	100ML	EUR 164

Trypsin-EDTA(10X), 0.5%
CA015-010	GenDepot	100ml	EUR 88

Trypsin-EDTA(10X), 0.5%
CA015-100	GenDepot	10x100ml	EUR 286

Trypsin-EDTA(10X), 0.5%
CA015-200	GenDepot	20x100ml	EUR 482

Trypsin-EDTA(10X), 0.05%
CA022-010	GenDepot	100ml	EUR 84

10x TAE Buffer Solution
BA01801	Bioatlas	6x100ml	EUR 97
Description: High purity buffer for various PCR applications.

10x PBS Buffer Solution
BA01802	Bioatlas	6x100ml	EUR 97
Description: High purity buffer for various PCR applications.

10x TBE Buffer Solution
BA01804	Bioatlas	6x100ml	EUR 97
Description: High purity buffer for various PCR applications.

10X TAE buffer solution
A00239	Bio Basic	500ml	EUR 56.36

RBC Lysis Solution 10X
abx290033-50ml	Abbexa	50 ml	EUR 230

TBST(10X), pH 7.4
T8056-100	GenDepot	1L	EUR 115

10x Gel Loading Buffer
TG4038	TopoGen	1ml	EUR 156

RBC Lysis Solution 10X
RBC10X-50ML	ImmunoStep	50 ML	EUR 108.5

10X KRB-IBMX Buffer
122937	Mediomics	1 Vial	EUR 77

Binding/Coating Buffer (10X)
85R-124	Fitzgerald	250 ml	EUR 216
Description: ELISA buffer for optimal coating and binding of antibodies and antigens

Alkaline Phosphate Buffer, 10x
K2191050-6	Biochain	50 ml	EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

TBS buffer (Tris-Buffered Saline), 20x Solution
UA0045	Bio Basic	500ml	EUR 66.97

T-Pro Fast Blocking Buffer (in TBS)
JK92-W002	T-Pro Biotechnology	500ml/BT	EUR 187

10x PBST Solution (10X Phosphate Buffered Saline, 0.5% Tween-20), PH 7.4
UPD8119	Bio Basic	500ml	EUR 67.4

Glutathione Reductase Conc., 10x, 1ML
X130-1ML	Arbor Assays	1ML	EUR 123

PBST buffer 10x Strength Solution
PW004	Bio Basic	500ml	EUR 67.4

TBST buffer 10x Strength Solution
PW009	Bio Basic	500ml	EUR 65.66

Annexin V Binding Buffer (10x)
EXB0019	ExBio	N/A	EUR 86

10x Taq Buffer (NH4)2SO4
PCRB55	Bio Basic	4x1.5ml, 6ml	EUR 58.7

SuperBlot Western Transfer buffer 10X
DG-WEST	Bio Basic	500ml	EUR 113.8

10X PBS Buffer , pH 7.4
P2100-050	GenDepot	500ml	EUR 88

10X PBS Buffer , pH 7.4
P2100-100	GenDepot	1L	EUR 91

Xpert Native Transfer Buffer(10X)
P9001-050	GenDepot	500ml	EUR 93

Xpert Native Transfer Buffer(10X)
P9001-100	GenDepot	1L	EUR 138

10x PBS Ready Mixed Powder
BA01701	Bioatlas	1L	EUR 68
Description: High purity buffer for various PCR applications.

10x TBE Ready Mixed Powder
BA01704	Bioatlas	1L	EUR 85
Description: High purity buffer for various PCR applications.

10x Tris-Glycine Buffer Solution
BA01806	Bioatlas	6x100ml	EUR 97
Description: High purity buffer for various PCR applications.

1 L TAE BUFFER, 10X
46-010-CM	CORNING	1L/pk	EUR 105
Description: Media Catalog; Classical Media

10X Fish Gelatin Blocking Agent
22010	Biotium	100ML	EUR 112
Description: Minimum order quantity: 1 unit of 100ML

Aniline, 10X in acetate buffer
91057	Biotium	10ML	EUR 111
Description: Minimum order quantity: 1 unit of 10ML

10X Tris-Glycine SDS buffer
T8053-050	GenDepot	500ml	EUR 85

10X Tris-Glycine SDS buffer
T8053-100	GenDepot	2X500ml	EUR 111

10X Tris-Glycine SDS buffer
T8053-101	GenDepot	1L	EUR 99

10X Tris-Glycine SDS buffer
T8053-104	GenDepot	4 L	EUR 153

10X Tris-Glycine SDS buffer
T8053-200	GenDepot	4X500ml	EUR 136

10X Tris-Glycine SDS buffer
T8053-201	GenDepot	2X1L	EUR 135

10X Tris-Glycine SDS buffer
T8053-401	GenDepot	4X1L	EUR 172

Topo I Assay Buffer 10x
TG4010	TopoGen	1ml	EUR 145

West-Ezier Washing Buffer(10X)
W3873-050	GenDepot	500ml	EUR 118

10X PBS MaxTag Histo (OKRA00043)
OKRA00043	Aviva Systems Biology	100 ml	EUR 140
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Citrate Buffer (10x) pH 6.0
CBB125	ScyTek Laboratories	125 ml	EUR 66

Citrate Buffer (10x) pH 6.0
CBB500	ScyTek Laboratories	500 ml	EUR 80

Citrate Buffer (10x) pH 6.0
CBB999	ScyTek Laboratories	1000 ml	EUR 98

Colloidal Gold Conjugate Diluent (10X)
CG-CD10	Alpha Diagnostics	10 ml	EUR 225

Colloidal Gold Running Buffer (10X)
CG-RB10	Alpha Diagnostics	10 ml	EUR 225

A high variable number of ROH, mean length and inbreeding values across chromosomes; positively the consequence of artificial selection. Pedigree-based inbreeding values tended to underestimate genomic-based inbreeding levels, which in turn varied depending on the method used for estimation.

The high positive correlations between different genomic-based inbreeding coefficients suggest that they are consistent and may be more accurate than pedigree-based methods, given that they capture information from past and more recent demographic events, even when there are no pedigree records available.