The characterization of runs of homozygosity (ROH), using high-density single nucleotide polymorphisms (SNPs) allows inferences to be made about the past demographic history of animal populations and the genomic ROH has become a common approach to characterize the inbreeding.
We aimed to analyze and characterize ROH patterns and compare different genomic and pedigree-based methods to estimate the inbreeding coefficient in two pure lines (POP A and B) and one recently admixed line (POP C) of coho salmon (Oncorhynchus kisutch) breeding nuclei, genotyped using a 200 K Affymetrix Axiom® myDesign Custom SNP Array.
A large number and greater mean length of ROH were found for the two “pure” lines and the recently admixed line (POP C) showed the lowest number and smaller mean length of ROH. The ROH analysis for different length classes suggests that all three coho salmon lines the genome is largely composed of a high number of short segments (<4 Mb), and for POP C no segment >16 Mb was found.
, 10x Solution) TBS buffer (Tris-Buffered Saline), 10x Solution |
|||
| A0027 | Bio Basic | 4L | EUR 93.93 |
TBS buffer (Tris-Buffered Saline), 10x Solution |
|||
| UA00279 | Bio Basic | 500ml | EUR 58.48 |
 10x pH 8.0, 1000 ml) Tris Buffered Saline (TBS) 10x pH 8.0, 1000 ml |
|||
| 12-9134-10 | Medicago | 1000 ml | EUR 459 |
 TBS |
|||
| I004 | Cygnus Technologies | 1000 ml | EUR 219 |
|
Description: TBS by Cygnus Technologies is available in Europe via Gentaur. |
|||
) T-Pro Washing buffer in TBS and Tween-20 (10X) |
|||
| JB09-I004 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
 for western blot washing) T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing |
|||
| JB09-I002 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
 concentrate (10X, 100 mM Tris/150mM NaCl final)) Tris Buffered Saline (TBS, pH 7.4) concentrate (10X, 100 mM Tris/150mM NaCl final) |
|||
| 80082 | Alpha Diagnostics | 1 L | EUR 225 |
 concentrate (10X, 50 mM Tris/150mM NaCl final)) Tris Buffered Saline (TBS, pH 7.4) concentrate (10X, 50 mM Tris/150mM NaCl final) |
|||
| 80083 | Alpha Diagnostics | 1 L | EUR 225 |
 Blocking Buffer) EZBlock? (TBS) Blocking Buffer |
|||
| 2117-1000 | Biovision | EUR 212 | |
EZBlock? (TBS) Blocking Buffer |
|||
| 2117-200 | Biovision | EUR 125 | |
 TBS Blocking Buffer Pack |
|||
| AR0143 | BosterBio | 200mL/pack | EUR 65 |
 TBS Wash Buffer Pack |
|||
| AR0144 | BosterBio | 500mL/pack | EUR 60 |
 TBS Wash Concentrate, 20X |
|||
| F004 | Cygnus Technologies | 50 ml | EUR 180 |
|
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur. |
|||
TBS Wash Concentrate, 20X |
|||
| F004-1000 | Cygnus Technologies | 1000 ml | EUR 403 |
|
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur. |
|||
 SkipDewax, 10X |
|||
| T213 | Insitus Biotechnologies | 50ml | EUR 297 |
 PBS, 10X |
|||
| UPD8117 | Bio Basic | 500ml | EUR 65.66 |
 THROMBOSTEP |
|||
| TBS-25 | ImmunoStep | 25 test | EUR 778 |
THROMBOSTEP |
|||
| TBS-50 | ImmunoStep | 50 test | EUR 986 |
 Blocking Buffer) EZBlock? T20 (TBS) Blocking Buffer |
|||
| 2140-1000 | Biovision | EUR 218 | |
EZBlock? T20 (TBS) Blocking Buffer |
|||
| 2140-200 | Biovision | EUR 120 | |
 Powder) Tris Buffered Saline (TBS) Powder |
|||
| AR0031 | BosterBio | 2000mL/pack | EUR 53 |
 TBS blocking buffer with BSA |
|||
| SD8113 | Bio Basic | 1pk, 1L | EUR 83.93 |
 TBS Buffer, 20X pH 7.4 |
|||
| T8054-050 | GenDepot | 500ml | EUR 86 |
TBS Buffer, 20X pH 7.4 |
|||
| T8054-101 | GenDepot | 1L | EUR 99 |
 TBS Buffer, 20X pH 7.6 |
|||
| T8055-050 | GenDepot | 500ml | EUR 86 |
TBS Buffer, 20X pH 7.6 |
|||
| T8055-101 | GenDepot | 1L | EUR 99 |
) PBS (10X Concentrate) |
|||
| 85R-125 | Fitzgerald | 250 ml | EUR 235 |
|
Description: Phosphate Buffered Saline (PBS) 10X concentrate |
|||
 TissueDigest Diluent, 10X |
|||
| T102 | Insitus Biotechnologies | 10ml | EUR 94 |
 450ML PBS 10X |
|||
| NAT1006 | National Diagnostics | 450ML | EUR 80 |
 4L PBS 10X |
|||
| NAT1008 | National Diagnostics | 4L | EUR 199 |
 1L TBE 10X |
|||
| NAT1196 | National Diagnostics | 1L | EUR 82 |
 4L TBE 10X |
|||
| NAT1198 | National Diagnostics | 4L | EUR 121 |
 10X Loading Buffer |
|||
| L0503-005 | GenDepot | 5X1ml | EUR 77 |
10X Loading Buffer |
|||
| L0504-005 | GenDepot | 5X1ml | EUR 77 |
 10X MOPS Buffer |
|||
| M1057-050 | GenDepot | 500ml | EUR 161 |
10X MOPS Buffer |
|||
| M1057-100 | GenDepot | 2X500ml | EUR 219 |
 NH4 Buffer, 10x |
|||
| BIO-37025 | Bioline | 3 x 1.2ml | Ask for price |
) Trypsin, 2.5%(10X) |
|||
| CA019-010 | GenDepot | 100ml | EUR 80 |
) Collagenase/Hyaluronidase (10X) |
|||
| CA094-002 | GenDepot | 20ml | EUR 340 |
 10x Taq Buffer |
|||
| BA00151 | Bioatlas | 1.8ml | EUR 55 |
|
Description: High purity buffer for various PCR applications. |
|||
 10X TAE Buffer |
|||
| T8048-101 | GenDepot | 1L | EUR 89 |
 10x Proteinase K |
|||
| TG4039 | TopoGen | 0.5ml | EUR 134 |
) ChonBlock Western Blot Buffer 1 (TBS) |
|||
| 9069T | Chondrex | 10 ml | EUR 193.55 |
|
Description: ChonBlock Western Blot Buffer 1 (TBS) |
|||
 Casein-based TBS Blocking Buffer Pack |
|||
| AR0183 | BosterBio | 500mL/Pack | EUR 70 |
, 10XpH 7.6) TBST (TBS-Tween 20), 10XpH 7.6 |
|||
| UA0046 | Bio Basic | 500ml | EUR 69.93 |
 TBS with non fat milk premix |
|||
| SD8104 | Bio Basic | 1pk | EUR 63.49 |
) Binding/Coating Buffer (10X) |
|||
| 85R-124 | Fitzgerald | 250 ml | EUR 216 |
|
Description: ELISA buffer for optimal coating and binding of antibodies and antigens |
|||
 10X KRB-IBMX Buffer |
|||
| 122937 | Mediomics | 1 Vial | EUR 77 |
 Blocking Buffer, 10X, 250ML |
|||
| X109-250ML | Arbor Assays | 250ML | EUR 359 |
 Blocking Buffer, 10X, 25ML |
|||
| X109-25ML | Arbor Assays | 25ML | EUR 122 |
 Alkaline Phosphate Buffer, 10x |
|||
| K2191050-6 | Biochain | 50 ml | EUR 137 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 10X TAE buffer solution |
|||
| A00239 | Bio Basic | 500ml | EUR 56.36 |
 RBC Lysis Solution 10X |
|||
| abx290033-50ml | Abbexa | 50 ml | EUR 230 |
 Tissue Pre-Conditioner, 10X |
|||
| T201 | Insitus Biotechnologies | 50ml | EUR 297 |
 SkipDewax Trial Size, 10X |
|||
| T212 | Insitus Biotechnologies | 10ml | EUR 123 |
 25ML 10X CE Buffer |
|||
| NAT1206 | National Diagnostics | 25ML | EUR 115 |
 100ML 10X CE Buffer |
|||
| NAT1208 | National Diagnostics | 100ML | EUR 164 |
, 0.5%) Trypsin-EDTA(10X), 0.5% |
|||
| CA015-010 | GenDepot | 100ml | EUR 88 |
Trypsin-EDTA(10X), 0.5% |
|||
| CA015-100 | GenDepot | 10x100ml | EUR 286 |
Trypsin-EDTA(10X), 0.5% |
|||
| CA015-200 | GenDepot | 20x100ml | EUR 482 |
, 0.05%) Trypsin-EDTA(10X), 0.05% |
|||
| CA022-010 | GenDepot | 100ml | EUR 84 |
 10x TAE Buffer Solution |
|||
| BA01801 | Bioatlas | 6x100ml | EUR 97 |
|
Description: High purity buffer for various PCR applications. |
|||
 10x PBS Buffer Solution |
|||
| BA01802 | Bioatlas | 6x100ml | EUR 97 |
|
Description: High purity buffer for various PCR applications. |
|||
 10x TBE Buffer Solution |
|||
| BA01804 | Bioatlas | 6x100ml | EUR 97 |
|
Description: High purity buffer for various PCR applications. |
|||
) 10x Taq Buffer (MgCl2) |
|||
| PCRB16 | Bio Basic | 4x1.5ml, 6ml | EUR 58.7 |
, pH 7.4) TBST(10X), pH 7.4 |
|||
| T8056-100 | GenDepot | 1L | EUR 115 |
 10x Gel Loading Buffer |
|||
| TG4038 | TopoGen | 1ml | EUR 156 |
RBC Lysis Solution 10X |
|||
| RBC10X-50ML | ImmunoStep | 50 ML | EUR 108.5 |
, 20x Solution) TBS buffer (Tris-Buffered Saline), 20x Solution |
|||
| UA0045 | Bio Basic | 500ml | EUR 66.97 |
) T-Pro Fast Blocking Buffer (in TBS) |
|||
| JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
, PH 7.4) 10x PBST Solution (10X Phosphate Buffered Saline, 0.5% Tween-20), PH 7.4 |
|||
| UPD8119 | Bio Basic | 500ml | EUR 67.4 |
 EIA Wash Buffer 10x concentrate |
|||
| 1003-1 | Ethos Biosciences | 1 Liter | EUR 231 |
 10X Annexin V Binding Buffer |
|||
| 1006-100 | Biovision | EUR 196 | |
 Glutathione Reductase Conc., 10x, 1ML |
|||
| X130-1ML | Arbor Assays | 1ML | EUR 123 |
 1 L TAE BUFFER, 10X |
|||
| 46-010-CM | CORNING | 1L/pk | EUR 105 |
|
Description: Media Catalog; Classical Media |
|||
 10X Fish Gelatin Blocking Agent |
|||
| 22010 | Biotium | 100ML | EUR 112 |
|
Description: Minimum order quantity: 1 unit of 100ML |
|||
) 10X Phosphate-Buffered Saline (PBS) |
|||
| 22020 | Biotium | 4L | EUR 200 |
|
Description: Minimum order quantity: 1 unit of 4L |
|||
 Aniline, 10X in acetate buffer |
|||
| 91057 | Biotium | 10ML | EUR 111 |
|
Description: Minimum order quantity: 1 unit of 10ML |
|||
 10X PBS Buffer , pH 7.4 |
|||
| P2100-050 | GenDepot | 500ml | EUR 88 |
10X PBS Buffer , pH 7.4 |
|||
| P2100-100 | GenDepot | 1L | EUR 91 |
) Xpert Native Transfer Buffer(10X) |
|||
| P9001-050 | GenDepot | 500ml | EUR 93 |
Xpert Native Transfer Buffer(10X) |
|||
| P9001-100 | GenDepot | 1L | EUR 138 |
 10x PBS Ready Mixed Powder |
|||
| BA01701 | Bioatlas | 1L | EUR 68 |
|
Description: High purity buffer for various PCR applications. |
|||
 10x TBE Ready Mixed Powder |
|||
| BA01704 | Bioatlas | 1L | EUR 85 |
|
Description: High purity buffer for various PCR applications. |
|||
 10x Tris-Glycine Buffer Solution |
|||
| BA01806 | Bioatlas | 6x100ml | EUR 97 |
|
Description: High purity buffer for various PCR applications. |
|||
 PBST buffer 10x Strength Solution |
|||
| PW004 | Bio Basic | 500ml | EUR 67.4 |
 TBST buffer 10x Strength Solution |
|||
| PW009 | Bio Basic | 500ml | EUR 65.66 |
) Annexin V Binding Buffer (10x) |
|||
| EXB0019 | ExBio | N/A | EUR 86 |
2SO4) 10x Taq Buffer (NH4)2SO4 |
|||
| PCRB55 | Bio Basic | 4x1.5ml, 6ml | EUR 58.7 |
 SuperBlot Western Transfer buffer 10X |
|||
| DG-WEST | Bio Basic | 500ml | EUR 113.8 |
 10X Tris-Glycine SDS buffer |
|||
| T8053-050 | GenDepot | 500ml | EUR 85 |
10X Tris-Glycine SDS buffer |
|||
| T8053-100 | GenDepot | 2X500ml | EUR 111 |
10X Tris-Glycine SDS buffer |
|||
| T8053-101 | GenDepot | 1L | EUR 99 |
10X Tris-Glycine SDS buffer |
|||
| T8053-104 | GenDepot | 4 L | EUR 153 |
10X Tris-Glycine SDS buffer |
|||
| T8053-200 | GenDepot | 4X500ml | EUR 136 |
10X Tris-Glycine SDS buffer |
|||
| T8053-201 | GenDepot | 2X1L | EUR 135 |
10X Tris-Glycine SDS buffer |
|||
| T8053-401 | GenDepot | 4X1L | EUR 172 |
 Topo I Assay Buffer 10x |
|||
| TG4010 | TopoGen | 1ml | EUR 145 |
) West-Ezier Washing Buffer(10X) |
|||
| W3873-050 | GenDepot | 500ml | EUR 118 |
 pH 6.0) Citrate Buffer (10x) pH 6.0 |
|||
| CBB125 | ScyTek Laboratories | 125 ml | EUR 66 |
Citrate Buffer (10x) pH 6.0 |
|||
| CBB500 | ScyTek Laboratories | 500 ml | EUR 80 |
Citrate Buffer (10x) pH 6.0 |
|||
| CBB999 | ScyTek Laboratories | 1000 ml | EUR 98 |
) Colloidal Gold Conjugate Diluent (10X) |
|||
| CG-CD10 | Alpha Diagnostics | 10 ml | EUR 225 |
A high variable number of ROH, mean length and inbreeding values across chromosomes; positively the consequence of artificial selection. Pedigree-based inbreeding values tended to underestimate genomic-based inbreeding levels, which in turn varied depending on the method used for estimation.
The high positive correlations between different genomic-based inbreeding coefficients suggest that they are consistent and may be more accurate than pedigree-based methods, given that they capture information from past and more recent demographic events, even when there are no pedigree records available.