enzymes in pcr

Insights into Genetic Diversity, Runs of Homozygosity and Heterozygosity-Rich Regions in Maremmana Semi-Feral Cattle Using Pedigree and Genomic Data

Posted by Norman Walker on in Antibodies, Assay Kits, Assessment of Autozygosity Derived From Runs of Homozygosity in Jinhua Pigs Disclosed by Sequencing Data., Autozygosity and Genetic Differentiation of Landrace and Large White Pigs as Revealed by the Genetic Analyses of Crossbreds., Biology Cells, Clia Kits, Culture Cells, Default, Devices, DNA, Effects of autozygosity and schizophrenia polygenic risk on cognitive and brain developmental trajectories., Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, NATtrol, Panel
Insights into Genetic Diversity, Runs of Homozygosity and Heterozygosity-Rich Regions in Maremmana Semi-Feral Cattle Using Pedigree and Genomic Data

Semi-feral native livestock populations, like Maremmana cattle, are the thing of renewed curiosity for the conservation of organic variety and the preservation and exploitation of distinctive and doubtlessly related genetic materials. The intention of this examine was to estimate genetic variety parameters in semi-feral Maremmana cattle utilizing each pedigree- and genomic-based approaches (FIS and FROH), and to detect areas of homozygosity (ROH) and heterozygosity (ROHet) in the genome.

The common heterozygosity estimates have been in the vary reported for different cattle breeds (HE=0.261, HO=0.274). Pedigree-based common inbreeding (F) was estimated at 4.9%. The correlation was low between F and genomic-based approaches (r=0.03 with FIS, r=0.21 with FROH), whereas it was increased between FIS and FROH (r=0.78).

The low correlation between F and FROH coefficients would be the end result of the restricted pedigree depth out there for the animals concerned in this examine. The ROH islands recognized in Maremmana cattle included candidate genes related to local weather adaptation, carcass traits or the regulation of physique weight, fats and vitality metabolism.

The ROHet islands contained candidate genes related to nematode resistance and replica traits in livestock. The outcomes of this examine affirm that genome-based measures like FROH could also be helpful estimators of particular person autozygosity, and could present insights on pedigree-based inbreeding estimates in instances when animals’ pedigree knowledge are unavailable, thus offering a extra detailed image of the genetic variety.

Inbreeding coefficients and runs of homozygosity islands in Brazilian water buffalo

Characterization of autozygosity is related to observe genetic variety and handle inbreeding ranges in breeding packages. Identification of autozygosity hotspots can unravel genomic areas focused by choice for economically necessary traits and may help establish candidate genes for choice. In this examine, we estimated the inbreeding ranges of a Brazilian inhabitants of Murrah buffalo present process choice for milk manufacturing traits, significantly milk yield.

We additionally studied the distribution of runs of homozygosity (ROH) islands and recognized putative genes and quantitative trait loci (QTL) beneath choice. We genotyped 422 Murrah buffalo for 51,611 SNP; 350 of these had ROH longer than 10 Mb, indicating the incidence of inbreeding in the final 5 generations.

The imply size of the ROH per animal was 4.28 ± 1.85 Mb. Inbreeding coefficients have been calculated from the genomic relationship matrix, the pedigree, and the ROH, with estimates various between 0.242 and 0.035. Inbreeding estimates from the pedigree had a low correlation with the genomic estimates, and estimates from the genomic relationship matrix have been a lot increased than these from the pedigree or the ROH.

Signatures of choice have been recognized in 6 genomic areas, positioned on chromosomes 1, 2, 3, 5, 16, and 18, encompassing a complete of 190 genes and 174 QTL. Many of the genes (e.g., APRT and ACSF3) and QTL recognized are associated to exploit manufacturing traits, comparable to milk yield, milk fats yield and proportion, and milk protein yield and proportion. Other genes are related to replica and immune response traits in addition to morphological points of the buffalo species.

Inbreeding ranges in this inhabitants are nonetheless low however are growing resulting from choice and needs to be managed to keep away from future losses resulting from inbreeding melancholy. The proximity of genes linked to exploit manufacturing traits with genes related to replica and immune system traits suggests the necessity to embody these latter genes in the breeding program to keep away from negatively affecting them resulting from choice for manufacturing traits.

Insights into Genetic Diversity, Runs of Homozygosity and Heterozygosity-Rich Regions in Maremmana Semi-Feral Cattle Using Pedigree and Genomic Data

Genome-wide evaluation of homozygosity areas in european simmental bulls

The examine of Runs of Homozygosity (ROH) is a helpful strategy for the characterization of the genome of livestock populations. Due to their excessive relationship with autozygosity, ROH enable to make inference about inhabitants genetic historical past, to estimate the extent of inbreeding, to evaluate inside breed heterogeneity and to detect the footprints of choice on livestock genomes.

Aim of this examine was to analyze the distribution of runs of homozygosity in bulls belonging to 5 European Simmental populations and to evaluate the connection between three manufacturing traits (milk yield, fats and protein contents) and autozygosity. ROH rely, distribution and ROH-based coefficient of inbreeding (FROH ) have been calculated for 3,845 Simmental bulls of 5 totally different European nations: Austria (AT), Switzerland (CH), Czech Republic (CZ), Germany (DE) and Italy (IT).

Carcinoembryonic Antigen (CEA)
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Description: A monoclonal antibody for detection of Carcinoembryonic Antigen from Human. This Carcinoembryonic Antigen antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

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EUR 289
Description: A monoclonal antibody for detection of Carcinoembryonic Antigen from Human. This Carcinoembryonic Antigen antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

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EUR 414
Description: A monoclonal antibody for detection of Carcinoembryonic Antigen from Human. This Carcinoembryonic Antigen antibody is for IHC-P, IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

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STJ96963 200 µl
EUR 197
Description: Carcinoembryonic antigen is a protein encoded by the CEACAM5 gene which is approximately 76,7 kDa. Carcinoembryonic antigen is localised to the cell membrane. It is involved in the metabolism of proteins and hematopoietic stem cell differentiation pathways and lineage-specific markers. It is a cell surface glycoprotein that plays a role in cell adhesion and in intracellular signalling. It may also regulate differentiation, apoptosis, and cell polarity and is used as a clinical biomarker for gastrointestinal cancers. Carcinoembryonic antigen is expressed in the intestine, liver, saliva, stomach and lung. Mutations in the CEACAM5 gene may be involved in urachal cancer. STJ96963 was developed from clone 10E1 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous Carcinoembryonic antigen proteins.

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Description: Recombinant Human Carcinoembryonic Antigen expressed in: 293F cell

Active Carcinoembryonic Antigen (CEA)
4-APA150Hu04
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Description: Recombinant Human Carcinoembryonic Antigen expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

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Description: Recombinant Human Carcinoembryonic Antigen expressed in: E.coli

Recombinant Carcinoembryonic Antigen (CEA)
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Description: Recombinant Human Carcinoembryonic Antigen expressed in: E.coli

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Description: Recombinant Human Carcinoembryonic Antigen expressed in: E.coli

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Description: Recombinant Human Carcinoembryonic Antigen expressed in: E.coli

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Description: Recombinant Mouse Carcinoembryonic Antigen expressed in: E.coli

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Description: Recombinant Mouse Carcinoembryonic Antigen expressed in: E.coli

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Description: Recombinant Rat Carcinoembryonic Antigen expressed in: E.coli

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  • 100 ug
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  • 100 ug
  • 10 ug
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  • EUR 704.00
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  • 100 ug
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Carcinoembryonic Antigen/CEA Monoclonal Antibody
EM1061-100ul 100ul
EUR 279
Description: A Mouse Monoclonal antibody against Carcinoembryonic Antigen/CEA from Human/ Rat/ Mouse. This antibody is tested and validated for IHC

Carcinoembryonic Antigen/CEA Monoclonal Antibody
EM1061-50ul 50ul
EUR 207
Description: A Mouse Monoclonal antibody against Carcinoembryonic Antigen/CEA from Human/ Rat/ Mouse. This antibody is tested and validated for IHC

Recombinant (Sf9) Human Carcinoembryonic Antigen
RP-755 10 ug
EUR 286

Human Carcinoembryonic Antigen (CEA) ELISA
LF-EK0161 1×96T
EUR 603

anti-Carcinoembryonic Antigen/CEA (10E1)
LF-MA20354 100 ug
EUR 354
Description: Mouse monoclonal to Carcinoembryonic Antigen (CEA)

Rat carcinoembryonic antigen ELISA kit
E02C1598-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Carcinoembryonic Antigen ELISA kit
E03C0348-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Carcinoembryonic Antigen ELISA kit
E03C0348-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Carcinoembryonic Antigen ELISA kit
E03C0348-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Carcinoembryonic Antigen ELISA kit
E08C0348-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Carcinoembryonic Antigen ELISA kit
E08C0348-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Carcinoembryonic Antigen ELISA kit
E08C0348-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog carcinoembryonic antigen ELISA kit
E08C1598-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog carcinoembryonic antigen ELISA kit
E08C1598-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog carcinoembryonic antigen ELISA kit
E08C1598-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Carcinoembryonic Antigen ELISA kit
E02C0348-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Carcinoembryonic Antigen ELISA kit
E02C0348-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Carcinoembryonic Antigen ELISA kit
E02C0348-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat carcinoembryonic antigen ELISA kit
E06C1598-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat carcinoembryonic antigen ELISA kit
E06C1598-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat carcinoembryonic antigen ELISA kit
E06C1598-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit carcinoembryonic antigen ELISA kit
E04C1598-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit carcinoembryonic antigen ELISA kit
E04C1598-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit carcinoembryonic antigen ELISA kit
E04C1598-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Carcinoembryonic Antigen ELISA kit
E04C0348-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Carcinoembryonic Antigen ELISA kit
E04C0348-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Carcinoembryonic Antigen ELISA kit
E04C0348-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit Carcinoembryonic Antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse carcinoembryonic antigen ELISA kit
E03C1598-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse carcinoembryonic antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Average values of ROH quantity per animal, and whole genome size lined by ROH have been 77.8 ± 20.7 and 205 ± 74.4 Mb, respectively. Bulls from AT, DE and IT exhibited comparable ROH traits. Swiss animals confirmed the best (12.6%), whereas CZ the bottom (4.6%) FROH coefficient.

The relationship between ROH incidence and milk manufacturing traits was investigated by means of a genome-wide ROH-traits affiliation evaluation (GWRA). A complete of 34 areas beforehand related to milk traits (yield and/or composition) have been recognized by GWRA.

Results of the current analysis spotlight a combined genetic background in the 5 European Simmental populations, with the doable presence of three subgroups. Moreover, a robust relationship between autozygosity and manufacturing traits has been detected.